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Immunohistochemical analysis of TNFα, <t>IL-12</t> and IL-23 in lung tissues of the bleomycin model of SSc mice. A Lung sections of sham treated (Control) or BLM treated (bleomycin model of SSc) WT or Capns1-ko mice were stained for TNFα, IL-23, IL-12 or FAP. Scale bars, 50 μm, magnification X200. (B, C, D,E) Mean staining area were quantified for TNFα B , IL-23 C , IL-12 D or FAP. E . The test of Homogeneity of variance showed P < 0.1, nonparametric test was applied. Data are mean ± SD. n = 10, *: P < 0.05, vs. sham treated WT mice; #: P < 0.05, vs. bleomycin model of SSc WT mice. OD : optical density
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Immunohistochemical analysis of TNFα, <t>IL-12</t> and IL-23 in lung tissues of the bleomycin model of SSc mice. A Lung sections of sham treated (Control) or BLM treated (bleomycin model of SSc) WT or Capns1-ko mice were stained for TNFα, IL-23, IL-12 or FAP. Scale bars, 50 μm, magnification X200. (B, C, D,E) Mean staining area were quantified for TNFα B , IL-23 C , IL-12 D or FAP. E . The test of Homogeneity of variance showed P < 0.1, nonparametric test was applied. Data are mean ± SD. n = 10, *: P < 0.05, vs. sham treated WT mice; #: P < 0.05, vs. bleomycin model of SSc WT mice. OD : optical density
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Immunohistochemical analysis of TNFα, IL-12 and IL-23 in lung tissues of the bleomycin model of SSc mice. A Lung sections of sham treated (Control) or BLM treated (bleomycin model of SSc) WT or Capns1-ko mice were stained for TNFα, IL-23, IL-12 or FAP. Scale bars, 50 μm, magnification X200. (B, C, D,E) Mean staining area were quantified for TNFα B , IL-23 C , IL-12 D or FAP. E . The test of Homogeneity of variance showed P < 0.1, nonparametric test was applied. Data are mean ± SD. n = 10, *: P < 0.05, vs. sham treated WT mice; #: P < 0.05, vs. bleomycin model of SSc WT mice. OD : optical density

Journal: Arthritis Research & Therapy

Article Title: Myeloid cell-specific deletion of Capns1 prevents macrophage polarization toward the M1 phenotype and reduces interstitial lung disease in the bleomycin model of systemic sclerosis

doi: 10.1186/s13075-022-02833-7

Figure Lengend Snippet: Immunohistochemical analysis of TNFα, IL-12 and IL-23 in lung tissues of the bleomycin model of SSc mice. A Lung sections of sham treated (Control) or BLM treated (bleomycin model of SSc) WT or Capns1-ko mice were stained for TNFα, IL-23, IL-12 or FAP. Scale bars, 50 μm, magnification X200. (B, C, D,E) Mean staining area were quantified for TNFα B , IL-23 C , IL-12 D or FAP. E . The test of Homogeneity of variance showed P < 0.1, nonparametric test was applied. Data are mean ± SD. n = 10, *: P < 0.05, vs. sham treated WT mice; #: P < 0.05, vs. bleomycin model of SSc WT mice. OD : optical density

Article Snippet: Rabbit anti-rat calpain1 polyclonal antibody (Abcam-28258, USA), rabbit anti-rat calpain2 polyclonal antibody (Abcam-236650, USA), rabbit anti-rat CD8 antibody (Santa Cruz Biotechnology-53212), rabbit anti-rat CD4 antibody (Abcam-207755, USA), rabbit anti-rat CD68 antibody (Abcam-125212, USA), rabbit anti-mouse Tumor necrosis factor-alpha (TNF-α) antibody (Santa Cruz Biotechnology-130349), rat anti-mouse F4/80 (BM8) monoclonal antibody (Santa Cruz Biotechnology-52664), mouse anti-human IL-10 (E-10) monoclonal antibody (Santa Cruz Biotechnology-8438), mouse anti-human IL-12 p70 (14L7) monoclonal antibody (Santa Cruz Biotechnology-74150), mouse anti-human IL-23 (D-12) monoclonal antibody (Santa Cruz Biotechnology-271349), COL3A1 (B-10) monoclonal antibody (Santa Cruz Biotechnology-271249), mouse anti-human COL1A1 (3G3) monoclonal antibody (Santa Cruz Biotechnology-293182), Rabbit anti-rat transforming growth factor-beta1 (TGF-β1(V)) polyclonal antibody (Santa Cruz Biotechnology-130348), Rabbit anti-rat MHC Class II polyclonal antibody (Huabio, ER 1913–01), Rabbit anti-rat fibroblast activation protein-α (FAP) polyclonal antibody (ABclonal, Catalog No.: A6349), α-SMA(Cell signaling technology-19245), CD206(Cell signaling technology-24595) were purchased for immunohistochemistry (IHC) and western blot (WB).

Techniques: Immunohistochemical staining, Staining